Hotspot

psbD-trnT-GGU

LSC · 34354-35539 · 1186 bp

Back to Myriopteris

Pi 0.0823

MarkerSeek score 87.10

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1186
variable_sites: 94
pi: 0.0823
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 87.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	GGACCAGCCCACAAATACAA	GCCCTTTTAACTCAGCGGTA	50.0	50.0	58.1	57.9	1339-1360 bp	1.0	86.7
psbD-trnT-GGU_p2	TTCTGAGCCAGTCGTCCATA	GCCCTTTTAACTCAGCGGTA	50.0	50.0	58.2	57.9	1310-1331 bp	1.0	86.3
psbD-trnT-GGU_p3	ATCTCTTCTGAGCCAGTCGT	GCCCTTTTAACTCAGCGGTA	50.0	50.0	58.2	57.9	1315-1336 bp	1.0	86.3
psbD-trnT-GGU_p4	ACGATCTCTTCTGAGCCAGT	GCCCTTTTAACTCAGCGGTA	50.0	50.0	58.2	57.9	1318-1339 bp	1.0	86.2
psbD-trnT-GGU_p5	GGACCAGCCCACAAATACAA	AAGCCCTTTTAACTCAGCGG	50.0	50.0	58.1	58.5	1341-1362 bp	1.0	83.8