Hotspot

trnY-GUA-trnE-UUC

LSC · 28027-28196 · 170 bp

Back to Myriopteris

Pi 0.061

MarkerSeek score 78.60

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 170
variable_sites: 10
pi: 0.061
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	ATTTGATTGGGTCGAGCTGG	CCAGGACACCTCTCTTTCAC	50.0	55.0	58.2	57.5	307-313 bp	1.0	78.5
trnY-GUA-trnE-UUC_p2	GATTGGGTCGAGCTGGATTC	CCAGGACACCTCTCTTTCAC	55.0	55.0	58.4	57.5	303-309 bp	1.0	77.2
trnY-GUA-trnE-UUC_p3	CTGGATTCGAACCAGCGTAG	CCAGGACACCTCTCTTTCAC	55.0	55.0	58.4	57.5	291-297 bp	1.0	77.1
trnY-GUA-trnE-UUC_p4	ATTTGATTGGGTCGAGCTGG	CCCAGGACACCTCTCTTTCA	50.0	55.0	58.2	58.6	308-314 bp	1.0	77.0
trnY-GUA-trnE-UUC_p5	GATTGGGTCGAGCTGGATTC	CCCAGGACACCTCTCTTTCA	55.0	55.0	58.4	58.6	304-310 bp	1.0	75.7