Hotspot

trnS-GCU-psb30

LSC · 8048-8753 · 706 bp

Back to Myriopteris

Pi 0.125
MarkerSeek score 82.60
Primer available yes
Label trnS-GCU-psb30

Feature Metadata

label_name
trnS-GCU-psb30
feature_type
igs
region
LSC
length_bp
706
variable_sites
87
pi
0.125
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-psb30_p1 GCAATCCGACGCTTTAAACC GCGAGTTGCGTAATTATTTCCA 50.0 40.9 58.1 57.7 771-806 bp 1.0 70.0
trnS-GCU-psb30_p2 TAAACCTCTCGGCCATCTCT GCGAGTTGCGTAATTATTTCCA 50.0 40.9 57.8 57.7 757-792 bp 1.0 69.6
trnS-GCU-psb30_p3 GCTTTAAACCTCTCGGCCAT GCGAGTTGCGTAATTATTTCCA 50.0 40.9 58.2 57.7 761-796 bp 1.0 68.9
trnS-GCU-psb30_p4 TCCGACGCTTTAAACCTCTC GCGAGTTGCGTAATTATTTCCA 50.0 40.9 57.6 57.7 767-802 bp 1.0 68.0
trnS-GCU-psb30_p5 GCAATCCGACGCTTTAAACC GCGAGTTGCGTAATTATTTCC 50.0 42.9 58.1 56.0 771-806 bp 1.0 64.5