primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	matK-rps16	1	CCTCAGAACCCAACTCGATG	GGGTTCATCTTTGCATGCAC	20	20	55.0	50.0	58.0	58.0	0.0	22.6	0.016165	2994	3700	1118-1118	0.500000	59.9
matK-rps16_p2	matK-rps16	2	CCTCAGAACCCAACTCGATG	GGTTCATCTTTGCATGCACC	20	20	55.0	50.0	58.0	58.0	0.0	22.6	0.016165	2994	3700	1117-1117	0.500000	59.9
matK-rps16_p3	matK-rps16	3	GTTTGGCCCATGTAATTGCC	GGGTTCATCTTTGCATGCAC	20	20	50.0	50.0	58.0	58.0	0.0	22.6	0.023194	2994	3700	1099-1099	0.500000	59.8
matK-rps16_p4	matK-rps16	4	GTTTGGCCCATGTAATTGCC	GGTTCATCTTTGCATGCACC	20	20	50.0	50.0	58.0	58.0	0.0	22.6	0.023194	2994	3700	1098-1098	0.500000	59.8
matK-rps16_p5	matK-rps16	5	CCTCAGAACCCAACTCGATG	CGCACGAACTGAAACTTTCC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.034453	2994	3700	1155-1155	0.500000	59.7
psbK-psbI_p1	psbK-psbI	1	CAAGCCGCAGTTAGTTTTCG	TCCAAAAACGAAAAGCGAAACA	20	22	50.0	36.4	58.0	58.2	0.0	0.0	2.214919	7224	7770	627-637	1.000000	70.9
psbK-psbI_p2	psbK-psbI	2	AGCCGCAGTTAGTTTTCGAT	TCCAAAAACGAAAAGCGAAACA	20	22	45.0	36.4	57.9	58.2	0.0	0.0	2.260152	7224	7770	625-635	1.000000	70.6
psbK-psbI_p3	psbK-psbI	3	AAGCCGCAGTTAGTTTTCGA	TCCAAAAACGAAAAGCGAAACA	20	22	45.0	36.4	58.1	58.2	0.0	0.0	2.291692	7224	7770	626-636	1.000000	70.3
psbK-psbI_p4	psbK-psbI	4	CAAGCCGCAGTTAGTTTTCG	CCAAAAACGAAAAGCGAAACA	20	21	50.0	38.1	58.0	56.8	0.0	0.0	2.298377	7224	7770	626-636	1.000000	70.2
psbK-psbI_p5	psbK-psbI	5	GCCAAGTTACCCGAAGCTTA	TCCAAAAACGAAAAGCGAAACA	20	22	50.0	36.4	57.9	58.2	0.0	0.0	2.268927	7224	7770	720-730	1.000000	69.4
trnS-GCU-psb30_p1	trnS-GCU-psb30	1	GCAATCCGACGCTTTAAACC	GCGAGTTGCGTAATTATTTCCA	20	22	50.0	40.9	58.1	57.7	0.0	0.0	2.376179	8048	8753	771-806	1.000000	70.0
trnS-GCU-psb30_p2	trnS-GCU-psb30	2	TAAACCTCTCGGCCATCTCT	GCGAGTTGCGTAATTATTTCCA	20	22	50.0	40.9	57.8	57.7	2.2	0.0	2.428078	8048	8753	757-792	1.000000	69.6
trnS-GCU-psb30_p3	trnS-GCU-psb30	3	GCTTTAAACCTCTCGGCCAT	GCGAGTTGCGTAATTATTTCCA	20	22	50.0	40.9	58.2	57.7	2.2	0.0	2.515920	8048	8753	761-796	1.000000	68.9
trnS-GCU-psb30_p4	trnS-GCU-psb30	4	TCCGACGCTTTAAACCTCTC	GCGAGTTGCGTAATTATTTCCA	20	22	50.0	40.9	57.6	57.7	0.0	0.0	2.626296	8048	8753	767-802	1.000000	68.0
trnS-GCU-psb30_p5	trnS-GCU-psb30	5	GCAATCCGACGCTTTAAACC	GCGAGTTGCGTAATTATTTCC	20	21	50.0	42.9	58.1	56.0	0.0	0.0	3.061337	8048	8753	771-806	1.000000	64.5
rpoB-trnD-GUC_p1	rpoB-trnD-GUC	1	TGCAAGTTCTGGTAGTACGG	GCGGTGCTCTAACCAATTGA	20	20	50.0	50.0	57.3	58.6	0.0	7.1	1.287159	26880	27741	917-920	1.000000	76.8
rpoB-trnD-GUC_p2	rpoB-trnD-GUC	2	GCAAGTTCTGGTAGTACGGT	GCGGTGCTCTAACCAATTGA	20	20	50.0	50.0	57.3	58.6	0.0	7.1	1.289028	26880	27741	916-919	1.000000	76.8
rpoB-trnD-GUC_p3	rpoB-trnD-GUC	3	TTCGTGCAAGTTCTGGTAGT	GCGGTGCTCTAACCAATTGA	20	20	45.0	50.0	57.1	58.6	0.0	7.1	1.453720	26880	27741	921-924	1.000000	75.5
rpoB-trnD-GUC_p4	rpoB-trnD-GUC	4	TCGTGCAAGTTCTGGTAGTAC	GCGGTGCTCTAACCAATTGA	21	20	47.6	50.0	57.7	58.6	0.0	7.1	1.844327	26880	27741	920-923	1.000000	72.4
rpoB-trnD-GUC_p5	rpoB-trnD-GUC	5	TGCAAGTTCTGGTAGTACGG	GGCGGTGCTCTAACCAATT	20	19	50.0	52.6	57.3	58.1	0.0	0.0	1.867891	26880	27741	918-921	1.000000	72.2
trnY-GUA-trnE-UUC_p1	trnY-GUA-trnE-UUC	1	ATTTGATTGGGTCGAGCTGG	CCAGGACACCTCTCTTTCAC	20	20	50.0	55.0	58.2	57.5	15.2	0.0	0.708978	28027	28196	307-313	1.000000	78.5
trnY-GUA-trnE-UUC_p2	trnY-GUA-trnE-UUC	2	GATTGGGTCGAGCTGGATTC	CCAGGACACCTCTCTTTCAC	20	20	55.0	55.0	58.4	57.5	15.2	0.0	0.876610	28027	28196	303-309	1.000000	77.2
trnY-GUA-trnE-UUC_p3	trnY-GUA-trnE-UUC	3	CTGGATTCGAACCAGCGTAG	CCAGGACACCTCTCTTTCAC	20	20	55.0	55.0	58.4	57.5	24.2	0.0	0.903234	28027	28196	291-297	1.000000	77.1
trnY-GUA-trnE-UUC_p4	trnY-GUA-trnE-UUC	4	ATTTGATTGGGTCGAGCTGG	CCCAGGACACCTCTCTTTCA	20	20	50.0	55.0	58.2	58.6	15.2	0.0	0.892737	28027	28196	308-314	1.000000	77.0
trnY-GUA-trnE-UUC_p5	trnY-GUA-trnE-UUC	5	GATTGGGTCGAGCTGGATTC	CCCAGGACACCTCTCTTTCA	20	20	55.0	55.0	58.4	58.6	15.2	0.0	1.060370	28027	28196	304-310	1.000000	75.7
psbM-petN_p1	psbM-petN	1	AAACAGCCGCTCGGAATAAC	CGTTTTCCCTCTCGCTTGTA	20	20	50.0	50.0	58.9	57.9	13.1	0.0	0.990258	28797	29723	997-999	1.000000	79.9
psbM-petN_p2	psbM-petN	2	AAACAGCCGCTCGGAATAAC	TACGTTTTCCCTCTCGCTTG	20	20	50.0	50.0	58.9	57.9	13.1	0.0	0.990258	28797	29723	999-1001	1.000000	79.9
psbM-petN_p3	psbM-petN	3	CAAACAGCCGCTCGGAATAA	CGTTTTCCCTCTCGCTTGTA	20	20	50.0	50.0	58.9	57.9	13.1	0.0	0.991058	28797	29723	998-1000	1.000000	79.9
psbM-petN_p4	psbM-petN	4	CAAACAGCCGCTCGGAATAA	TACGTTTTCCCTCTCGCTTG	20	20	50.0	50.0	58.9	57.9	13.1	0.0	0.991058	28797	29723	1000-1002	1.000000	79.9
psbM-petN_p5	psbM-petN	5	TACATTCAAACAGCCGCTCG	CGTTTTCCCTCTCGCTTGTA	20	20	50.0	50.0	58.9	57.9	0.0	0.0	0.999005	28797	29723	1004-1006	1.000000	79.8
psbD-trnT-GGU_p1	psbD-trnT-GGU	1	GGACCAGCCCACAAATACAA	GCCCTTTTAACTCAGCGGTA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.185665	34354	35539	1339-1360	1.000000	86.7
psbD-trnT-GGU_p2	psbD-trnT-GGU	2	TTCTGAGCCAGTCGTCCATA	GCCCTTTTAACTCAGCGGTA	20	20	50.0	50.0	58.2	57.9	2.7	0.0	0.256209	34354	35539	1310-1331	1.000000	86.3
psbD-trnT-GGU_p3	psbD-trnT-GGU	3	ATCTCTTCTGAGCCAGTCGT	GCCCTTTTAACTCAGCGGTA	20	20	50.0	50.0	58.2	57.9	2.7	0.0	0.257531	34354	35539	1315-1336	1.000000	86.3
psbD-trnT-GGU_p4	psbD-trnT-GGU	4	ACGATCTCTTCTGAGCCAGT	GCCCTTTTAACTCAGCGGTA	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.257531	34354	35539	1318-1339	1.000000	86.2
psbD-trnT-GGU_p5	psbD-trnT-GGU	5	GGACCAGCCCACAAATACAA	AAGCCCTTTTAACTCAGCGG	20	20	50.0	50.0	58.1	58.5	0.0	0.0	0.547399	34354	35539	1341-1362	1.000000	83.8
trnS-GGA-rps4_p1	trnS-GGA-rps4	1	GTTCGAATCCCTCCCCCT	TGAGTATCATTCCCGAAAAGCT	18	22	61.1	40.9	57.9	57.5	0.0	0.0	4.555972	43605	44054	488-496	1.000000	52.9
trnS-GGA-rps4_p2	trnS-GGA-rps4	2	GTTCGAATCCCTCCCCCTC	TGAGTATCATTCCCGAAAAGCT	19	22	63.2	40.9	59.2	57.5	0.0	0.0	4.656879	43605	44054	488-496	1.000000	52.0
trnS-GGA-rps4_p3	trnS-GGA-rps4	3	TTCGAATCCCTCCCCCTC	TGAGTATCATTCCCGAAAAGCT	18	22	61.1	40.9	57.6	57.5	0.0	0.0	4.881816	43605	44054	487-495	1.000000	50.2
trnS-GGA-rps4_p4	trnS-GGA-rps4	4	GTTCGAATCCCTCCCCCT	TGAGTATCATTCCCGAAAAGC	18	21	61.1	42.9	57.9	56.0	0.0	0.0	5.023147	43605	44054	488-496	1.000000	49.3
trnS-GGA-rps4_p5	trnS-GGA-rps4	5	GTTCGAATCCCTCCCCCTC	TGAGTATCATTCCCGAAAAGC	19	21	63.2	42.9	59.2	56.0	0.0	0.0	5.124054	43605	44054	488-496	1.000000	49.3
petA-psbJ_p1	petA-psbJ	1	TGTTGGGGGATTTGGTCAAG	TTAGCTTACGACGTTTCCGG	20	20	50.0	50.0	58.0	58.0	0.0	5.5	0.026989	59649	60660	1825-1834	1.000000	86.5
petA-psbJ_p2	petA-psbJ	2	TGTTGGGGGATTTGGTCAAG	GGCTGGCTGTCTGTAAGTAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.019783	59649	60660	1849-1858	1.000000	86.4
petA-psbJ_p3	petA-psbJ	3	GCAGATCAGCCGTTGACTAA	GGCTGGCTGTCTGTAAGTAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.021685	59649	60660	1878-1887	1.000000	86.4
petA-psbJ_p4	petA-psbJ	4	GCAGATCAGCCGTTGACTAA	TTAGCTTACGACGTTTCCGG	20	20	50.0	50.0	58.0	58.0	0.0	5.5	0.028891	59649	60660	1854-1863	1.000000	86.4
petA-psbJ_p5	petA-psbJ	5	CAGCGACGGGAAGGATAAAA	GGCTGGCTGTCTGTAAGTAC	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.030082	59649	60660	2027-2036	1.000000	86.1
clpP1-psbB_p1	clpP1-psbB	1	CGAAAGGGCACTTTTGGAAC	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	57.9	58.2	19.5	0.0	0.371494	67908	68415	558-560	1.000000	83.3
clpP1-psbB_p2	clpP1-psbB	2	CTATACGTCAACCCGAACCG	CGATACCAAGGCAAACCCAT	20	20	55.0	50.0	58.2	58.2	0.0	0.0	0.405950	67908	68415	598-600	1.000000	82.6
clpP1-psbB_p3	clpP1-psbB	3	CGAAAGGGCACTTTTGGAAC	TAATACGACGGTGTGAACGC	20	20	50.0	50.0	57.9	58.4	19.5	0.0	0.517581	67908	68415	577-579	1.000000	81.9
clpP1-psbB_p4	clpP1-psbB	4	CGACATACCTAACGAGTCGC	CGATACCAAGGCAAACCCAT	20	20	55.0	50.0	58.3	58.2	13.6	0.0	0.497675	67908	68415	619-621	1.000000	81.7
clpP1-psbB_p5	clpP1-psbB	5	GCACTATACGTCAACCCGAA	CGATACCAAGGCAAACCCAT	20	20	50.0	50.0	57.7	58.2	0.0	0.0	0.519993	67908	68415	601-603	1.000000	81.7
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	GTCCAACGCGACTTGATTTG	AATCCTGGTCGTTGAATCGG	20	20	50.0	50.0	58.0	58.0	15.9	0.0	0.073704	105969	108547	2985-2985	0.500000	59.4
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	GTCCAACGCGACTTGATTTG	TTTCAAGTTTTGATCGGCGC	20	20	50.0	45.0	58.0	58.0	15.9	0.0	0.094416	105969	108547	2901-2901	0.500000	59.2
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	CCTCCACTTGTTGGTGTTGA	TTTCAAGTTTTGATCGGCGC	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.096222	105969	108547	2970-2970	0.500000	59.2
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	TCCACTTGTTGGTGTTGAGG	TTTCAAGTTTTGATCGGCGC	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.096222	105969	108547	2968-2968	0.500000	59.2
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	GTCCAACGCGACTTGATTTG	AATCTTTTTGCAGGGGAGGG	20	20	50.0	50.0	58.0	58.1	15.9	0.0	0.106752	105969	108547	2845-2845	0.500000	59.1
rpl32-trnP-GGG_p1	rpl32-trnP-GGG	1	ATCTTACACAGCTGCTTCGG	CTCTGGCACTGCTTCCTAAG	20	20	50.0	55.0	58.0	58.0	15.3	0.0	0.037091	111812	112417	1468-1468	0.500000	59.7
rpl32-trnP-GGG_p2	rpl32-trnP-GGG	2	ATCTTACACAGCTGCTTCGG	CTGGCACTGCTTCCTAAGAG	20	20	50.0	55.0	58.0	58.0	15.3	0.0	0.037091	111812	112417	1466-1466	0.500000	59.7
rpl32-trnP-GGG_p3	rpl32-trnP-GGG	3	CCGAATCTTACACAGCTGCT	CTCTGGCACTGCTTCCTAAG	20	20	50.0	55.0	58.0	58.0	15.3	0.0	0.037091	111812	112417	1472-1472	0.500000	59.7
rpl32-trnP-GGG_p4	rpl32-trnP-GGG	4	CCGAATCTTACACAGCTGCT	CTGGCACTGCTTCCTAAGAG	20	20	50.0	55.0	58.0	58.0	15.3	0.0	0.037091	111812	112417	1470-1470	0.500000	59.7
rpl32-trnP-GGG_p5	rpl32-trnP-GGG	5	GCAAATCCGTAGAGAAGGCA	CTGGCACTGCTTCCTAAGAG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.045531	111812	112417	1067-1067	0.500000	59.6
trnP-GGG-trnL-UAG_p1	trnP-GGG-trnL-UAG	1	TGAACCTGTGACCTCCATCA	AGAGCAGCGTGTCTACCTAT	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.331727	112492	112679	272-280	1.000000	86.3
trnP-GGG-trnL-UAG_p2	trnP-GGG-trnL-UAG	2	TGAACCTGTGACCTCCATCA	CTCTGGCACTGCTTCCTAAG	20	20	50.0	55.0	58.3	58.0	0.0	0.0	0.293032	112492	112679	290-298	1.000000	86.1
trnP-GGG-trnL-UAG_p3	trnP-GGG-trnL-UAG	3	TGAACCTGTGACCTCCATCA	CTGGCACTGCTTCCTAAGAG	20	20	50.0	55.0	58.3	58.0	0.0	0.0	0.293032	112492	112679	288-296	1.000000	86.1
trnP-GGG-trnL-UAG_p4	trnP-GGG-trnL-UAG	4	TGAACCTGTGACCTCCATCA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.3	57.9	0.0	0.0	0.395954	112492	112679	277-285	1.000000	85.7
trnP-GGG-trnL-UAG_p5	trnP-GGG-trnL-UAG	5	TGAACCTGTGACCTCCATCA	AAGAGCAGCGTGTCTACCTA	20	20	50.0	50.0	58.3	58.2	0.0	0.0	0.436182	112492	112679	273-281	1.000000	85.5
ndhD-psaC_p1	ndhD-psaC	1	TAGCGTTAGCCGAGGTACAT	GGATCTGAAACTACCCGCAG	20	20	50.0	55.0	58.3	58.1	0.0	0.0	0.374732	115727	115893	224-236	1.000000	89.0
ndhD-psaC_p2	ndhD-psaC	2	GCGTTAGCCGAGGTACATTA	GGATCTGAAACTACCCGCAG	20	20	50.0	55.0	57.5	58.1	0.0	0.0	0.555782	115727	115893	222-234	1.000000	87.6
ndhD-psaC_p3	ndhD-psaC	3	TTGGGAAGGAAACGACTAGC	GGATCTGAAACTACCCGCAG	20	20	50.0	55.0	57.5	58.1	0.0	0.0	0.526241	115727	115893	240-252	1.000000	87.5
ndhD-psaC_p4	ndhD-psaC	4	GACTAGCGTTAGCCGAGGTA	GGATCTGAAACTACCCGCAG	20	20	55.0	55.0	58.7	58.1	6.2	0.0	0.755366	115727	115893	227-239	1.000000	86.0
ndhD-psaC_p5	ndhD-psaC	5	TAGCGTTAGCCGAGGTACAT	GATCTGAAACTACCCGCAGT	20	20	50.0	50.0	58.3	57.3	0.0	0.0	0.990852	115727	115893	223-235	1.000000	84.1
chlL-trnN-GUU_p1	chlL-trnN-GUU	1	TCCATAAACTGCTATTTTCGT	AGGATTCGAACCTACGACCA	21	20	33.3	50.0	53.2	58.2	0.0	15.0	5.912979	129964	130708	539-828	1.000000	52.0
chlL-trnN-GUU_p2	chlL-trnN-GUU	2	TTCCATAAACTGCTATTTTCGT	AGGATTCGAACCTACGACCA	22	20	31.8	50.0	54.1	58.2	0.0	15.0	6.050835	129964	130708	540-829	1.000000	52.0
chlL-trnN-GUU_p3	chlL-trnN-GUU	3	TCCATAAACTGCTATTTTCGT	CCGGGTAGGATTCGAACCTA	21	20	33.3	55.0	53.2	58.3	0.0	17.3	6.060284	129964	130708	545-834	1.000000	52.0
chlL-trnN-GUU_p4	chlL-trnN-GUU	4	TTCCATAAACTGCTATTTTCGT	CCGGGTAGGATTCGAACCTA	22	20	31.8	55.0	54.1	58.3	0.0	17.3	6.198140	129964	130708	546-835	1.000000	52.0
chlL-trnN-GUU_p5	chlL-trnN-GUU	5	TTTCCATAAACTGCTATTTTCGT	AGGATTCGAACCTACGACCA	23	20	30.4	50.0	54.9	58.2	0.0	15.0	6.263151	129964	130708	541-830	1.000000	52.0