Hotspot

ycf1

SSC · 130391-135709 · 5319 bp

Back to Myriophyllum

Pi 0.0712

MarkerSeek score 77.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5319
variable_sites: 756
pi: 0.0712
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTTCGGGGATGACCAAATGG	GGTTGAAAGAAATGCCCAGC	50.0	50.0	58.2	57.9	648-663 bp	0.6	71.9
ycf1_p2	TTTCGGGGATGACCAAATGG	GTTGAAAGAAATGCCCAGCC	50.0	50.0	58.2	57.9	647-662 bp	0.6	71.9
ycf1_p3	TGGAACTTCGCTTTCCTGTC	GGTTGAAAGAAATGCCCAGC	50.0	50.0	58.1	57.9	637-646 bp	0.4	57.1
ycf1_p4	TGGAACTTCGCTTTCCTGTC	GTTGAAAGAAATGCCCAGCC	50.0	50.0	58.1	57.9	636-645 bp	0.4	57.1
ycf1_p5	AGAAGAAGGTCGTTTTCGGG	GTTGAAAGAAATGCCCAGCC	50.0	50.0	57.8	57.9	666-675 bp	0.4	56.7