Hotspot

rpl16

LSC · 86407-88121 · 1715 bp

Back to Myriophyllum

Pi 0.0583

MarkerSeek score 82.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1715
variable_sites: 172
pi: 0.0583
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CGCACCAAAAACTCGAGTTC	GCCGAATTTATAGCCGGACA	50.0	50.0	58.0	58.1	2388-2572 bp	0.8	81.3
rpl16_p2	CGCACCAAAAACTCGAGTTC	TGCCGAATTTATAGCCGGAC	50.0	50.0	58.0	58.1	2389-2573 bp	0.8	81.3
rpl16_p3	CGCACCAAAAACTCGAGTTC	GTGTCGAATGGAGCAGAGAG	50.0	55.0	58.0	58.1	2301-2430 bp	0.6	70.8
rpl16_p4	CGCACCAAAAACTCGAGTTC	GTTCTGTTCGATCCAGTGCT	50.0	50.0	58.0	57.9	2151-2280 bp	0.6	70.8
rpl16_p5	GCGTACAATTACAGCTCGGA	GTGTCGAATGGAGCAGAGAG	50.0	55.0	58.1	58.1	2190-2319 bp	0.6	70.4