Hotspot

rps3-rps19

LSC · 88938-89664 · 727 bp

Back to Myriophyllum

Pi 0.0527
MarkerSeek score 84.70
Primer available yes
Label rps3-rps19

Feature Metadata

label_name
rps3-rps19
feature_type
igs
region
LSC
length_bp
727
variable_sites
75
pi
0.0527
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
84.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps3-rps19_p1 CTCTCTGCTCCATTCGACAC GGGTAGAGCCGGATCTAAGT 55.0 55.0 58.1 58.0 1785-1863 bp 1.0 87.7
rps3-rps19_p2 CTCTCTGCTCCATTCGACAC TGGGTAGAGCCGGATCTAAG 55.0 55.0 58.1 58.0 1786-1864 bp 1.0 87.7
rps3-rps19_p3 CTCTCTGCTCCATTCGACAC AACCCTGTAGACCATCCACA 55.0 50.0 58.1 58.0 1724-1802 bp 1.0 87.7
rps3-rps19_p4 AGCACTGGATCGAACAGAAC GGGTAGAGCCGGATCTAAGT 50.0 55.0 57.9 58.0 1998-2013 bp 0.6 65.6
rps3-rps19_p5 AGCACTGGATCGAACAGAAC TGGGTAGAGCCGGATCTAAG 50.0 55.0 57.9 58.0 1999-2014 bp 0.6 65.6