Hotspot

psaA-ycf3

LSC · 45386-46620 · 1235 bp

Back to Myriophyllum

Pi 0.0941

MarkerSeek score 76.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 1235
variable_sites: 162
pi: 0.0941
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	ATGCCACTCAGCCAAAGAAA	GAATTGACTGCTGAGCCGTA	45.0	50.0	58.0	58.0	1344-1730 bp	1.0	91.9
psaA-ycf3_p2	ATTTGGGAGCAGCTTTGTGA	GAATTGACTGCTGAGCCGTA	45.0	50.0	58.0	58.0	1634-2020 bp	1.0	91.9
psaA-ycf3_p3	TTTGGGAGCAGCTTTGTGAT	GAATTGACTGCTGAGCCGTA	45.0	50.0	58.0	58.0	1633-2019 bp	1.0	91.9
psaA-ycf3_p4	ATGCCACTCAGCCAAAGAAA	CTGAGCCGTATGAGGTAGGA	45.0	55.0	58.0	58.0	1334-1720 bp	1.0	91.9
psaA-ycf3_p5	TTTGGGAGCAGCTTTGTGAT	CTGAGCCGTATGAGGTAGGA	45.0	55.0	58.0	58.0	1623-2009 bp	1.0	91.8