Hotspot

lhbA-trnG-UCC

LSC · 39008-39991 · 984 bp

Back to Myriophyllum

Pi 0.122
MarkerSeek score 79.90
Primer available yes
Label lhbA-trnG-UCC

Feature Metadata

label_name
lhbA-trnG-UCC
feature_type
igs
region
LSC
length_bp
984
variable_sites
202
pi
0.122
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.89
markerseek_score
79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
lhbA-trnG-UCC_p1 GGTATCCTTAATTCTCTCATTTCTTGA TACCATTCAACCATATCCGC 33.3 45.0 57.2 55.3 751-1031 bp 1.0 52.0
lhbA-trnG-UCC_p2 TCCTTAATTCTCTCATTTCTTGA TACCATTCAACCATATCCGC 30.4 45.0 53.1 55.3 747-1027 bp 1.0 52.0
lhbA-trnG-UCC_p3 GGTATCCTTAATTCTCTCATTTCTTGA TTACCATTCAACCATATCCGC 33.3 42.9 57.2 56.1 752-1032 bp 1.0 52.0
lhbA-trnG-UCC_p4 GGTATCCTTAATTCTCTCATTTCTTG TACCATTCAACCATATCCGC 34.6 45.0 55.9 55.3 751-1031 bp 1.0 52.0
lhbA-trnG-UCC_p5 TCCTTAATTCTCTCATTTCTTGA TTACCATTCAACCATATCCGC 30.4 42.9 53.1 56.1 748-1028 bp 1.0 52.0