Hotspot

trnC-GCA-trnT-GGU

LSC · 32975-34206 · 1232 bp

Back to Myriophyllum

Pi 0.0918

MarkerSeek score 82.30

Primer available yes

Label trnC-GCA-trnT-GGU

Feature Metadata

label_name: trnC-GCA-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1232
variable_sites: 194
pi: 0.0918
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-trnT-GGU_p1	CCGGATTTGAACTGGGGAAA	GAACCGATGACTTACGCCTT	50.0	50.0	58.1	58.0	1200-1376 bp	1.0	91.3
trnC-GCA-trnT-GGU_p2	CCGGATTTGAACTGGGGAAA	TCGATGGGTTAGGTCCACTT	50.0	50.0	58.1	58.1	1591-1752 bp	1.0	90.9
trnC-GCA-trnT-GGU_p3	CCGGATTTGAACTGGGGAAA	TTCGATGGGTTAGGTCCACT	50.0	50.0	58.1	58.1	1592-1753 bp	1.0	90.9
trnC-GCA-trnT-GGU_p4	AGCCCAAGCGAGACTTACTA	GAACCGATGACTTACGCCTT	50.0	50.0	58.2	58.0	1672-1817 bp	1.0	90.7
trnC-GCA-trnT-GGU_p5	GTGTTCTGACCCAACACGAT	GAACCGATGACTTACGCCTT	50.0	50.0	58.1	58.0	1689 bp	0.2	47.0