Hotspot

rpoB-trnE-UUC

LSC · 29139-29735 · 597 bp

Back to Myriophyllum

Pi 0.0782

MarkerSeek score 83.90

Primer available yes

Label rpoB-trnE-UUC

Feature Metadata

label_name: rpoB-trnE-UUC
feature_type: igs
region: LSC
length_bp: 597
variable_sites: 83
pi: 0.0782
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnE-UUC_p1	ATGGTCCAATGCTTTTCGGT	ATCAGCAAATTTTTGGGCCG	45.0	45.0	58.1	57.9	1208-1233 bp	0.6	66.4
rpoB-trnE-UUC_p2	ATGGTCCAATGCTTTTCGGT	AAATTTTGTCCCCTGTCCCC	45.0	50.0	58.1	58.0	1612 bp	0.2	47.2
rpoB-trnE-UUC_p3	CCCCACCTACACAAGCAAAT	AAATTTTGTCCCCTGTCCCC	50.0	50.0	58.1	58.0	1898 bp	0.2	47.2
rpoB-trnE-UUC_p4	CGTCAAGCCCTGATCAAGAA	AAATTTTGTCCCCTGTCCCC	50.0	50.0	57.9	58.0	1282 bp	0.2	47.0
rpoB-trnE-UUC_p5	GACTCACACGAGCCCATATC	AAATTTTGTCCCCTGTCCCC	55.0	50.0	57.9	58.0	1712 bp	0.2	46.7