Hotspot

rpl32-trnL-UAG

SSC · 113538-114931 · 1394 bp

Back to Myricaria

Pi 0.0186

MarkerSeek score 70.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1394
variable_sites: 65
pi: 0.0186
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGAAGGGATATTGGGTAGCG	TCCTAAGAGCAGCGTGTCTA	55.0	50.0	57.5	57.9	1484-1529 bp	1.0	82.6
rpl32-trnL-UAG_p2	GGAAGGGATATTGGGTAGCG	AGCAGCGTGTCTACCAATTT	55.0	45.0	57.5	57.8	1477-1522 bp	1.0	82.2
rpl32-trnL-UAG_p3	GGAAGGGATATTGGGTAGCG	CCTAAGAGCAGCGTGTCTAC	55.0	55.0	57.5	57.8	1483-1528 bp	1.0	82.1
rpl32-trnL-UAG_p4	GGAAGGGATATTGGGTAGCG	CTAAGAGCAGCGTGTCTACC	55.0	55.0	57.5	57.8	1482-1527 bp	1.0	82.1
rpl32-trnL-UAG_p5	GGAAGGGATATTGGGTAGCG	GAGCAGCGTGTCTACCAATT	55.0	50.0	57.5	58.3	1478-1523 bp	1.0	81.5