Hotspot

ndhF-rpl32

SSC · 112683-113387 · 705 bp

Back to Myricaria

Pi 0.0185

MarkerSeek score 65.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 705
variable_sites: 29
pi: 0.0185
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AGGTATGATCCATGCATATTCGT	CGCTACCCAATATCCCTTCC	39.1	55.0	57.6	57.5	813-880 bp	1.0	54.2
ndhF-rpl32_p2	GGTATGATCCATGCATATTCGT	CGCTACCCAATATCCCTTCC	40.9	55.0	56.2	57.5	812-879 bp	1.0	50.9
ndhF-rpl32_p3	AGGTATGATCCATGCATATTCG	CGCTACCCAATATCCCTTCC	40.9	55.0	55.9	57.5	813-880 bp	1.0	48.7
ndhF-rpl32_p4	AGGTATGATCCATGCATATTCGT	ACGCTACCCAATATCCCTTC	39.1	50.0	57.6	56.8	814-881 bp	1.0	48.2
ndhF-rpl32_p5	AAGGTATGATCCATGCATATTCGT	CGCTACCCAATATCCCTTCC	37.5	55.0	58.2	57.5	814-881 bp	1.0	48.1