Hotspot

psbE-petL

LSC · 65284-66011 · 728 bp

Back to Myricaria

Pi 0.0092

MarkerSeek score 64.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 728
variable_sites: 15
pi: 0.0092
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GCCGCTAGTAAGAAACCGAA	50.0	50.0	57.9	58.0	722-879 bp	1.0	85.0
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AAGCCGCTAGTAAGAAACCG	50.0	50.0	57.9	58.0	724-881 bp	1.0	84.9
psbE-petL_p3	AGAACGTTCTCCTGTGCTTC	GCCGCTAGTAAGAAACCGAA	50.0	50.0	57.8	58.0	645-802 bp	1.0	84.3
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AAGCCGCTAGTAAGAAACCG	50.0	50.0	57.8	58.0	647-804 bp	1.0	84.2
psbE-petL_p5	ACGAATAACCAACCCGCAAT	GCCGCTAGTAAGAAACCGAA	45.0	50.0	58.2	58.0	731-888 bp	1.0	84.0