Hotspot

ndhC-trnV-UAC

LSC · 51819-52650 · 832 bp

Back to Myricaria

Pi 0.0124
MarkerSeek score 71.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
832
variable_sites
25
pi
0.0124
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GTCCTTTGCTAATCGGAGCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 893-996 bp 1.0 81.9
ndhC-trnV-UAC_p2 GTCCTTTGCTAATCGGAGCT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.7 57.5 892-995 bp 1.0 79.2
ndhC-trnV-UAC_p3 CTGGTCCTTTGCTAATCGGA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 896-999 bp 1.0 78.9
ndhC-trnV-UAC_p4 TCTGGTCCTTTGCTAATCGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 897-1000 bp 1.0 78.9
ndhC-trnV-UAC_p5 TGGTCCTTTGCTAATCGGAG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 895-998 bp 1.0 78.9