Hotspot

trnF-GAA-ndhJ

LSC · 49394-50138 · 745 bp

Back to Myricaria

Pi 0.0142

MarkerSeek score 62.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 745
variable_sites: 27
pi: 0.0142
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	883-900 bp	1.0	82.7
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	847-864 bp	1.0	82.4
trnF-GAA-ndhJ_p3	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	871-888 bp	1.0	81.7
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	ATGCCCGAAAGTTGGATAGG	55.0	50.0	58.0	57.7	892-909 bp	1.0	80.6
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATGCCCGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	856-873 bp	1.0	80.3