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rps4-trnT-UGU

LSC · 46946-47378 · 433 bp

Back to Myricaria

Pi 0.0139

MarkerSeek score 61.40

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 433
variable_sites: 16
pi: 0.0139
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TCACTTCCACCTTTAGGCCT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.3	57.9	588-602 bp	1.0	80.8
rps4-trnT-UGU_p2	TCACTTCCACCTTTAGGCCT	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.3	57.8	556-570 bp	1.0	80.1
rps4-trnT-UGU_p3	CACTTCCACCTTTAGGCCTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.4	57.9	587-601 bp	1.0	78.2
rps4-trnT-UGU_p4	TCACTTCCACCTTTAGGCCT	ATCAGCCTGCTTAGCTCAGA	50.0	50.0	58.3	58.5	602-616 bp	1.0	77.7
rps4-trnT-UGU_p5	TCACTTCCACCTTTAGGCCT	CAGAGGTTAGAGCATCGCAT	50.0	50.0	58.3	57.5	586-600 bp	1.0	77.7