Hotspot

ycf3-trnS-GGA

LSC · 45293-45997 · 705 bp

Back to Myricaria

Pi 0.0113

MarkerSeek score 63.70

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 705
variable_sites: 22
pi: 0.0113
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTGGCCACGATTGAAAAGGT	TGCTACGCCTTTAACCACTC	45.0	50.0	58.3	57.9	794-810 bp	1.0	79.3
ycf3-trnS-GGA_p2	TTGGCCACGATTGAAAAGGT	CCCTCGGTAAACAAAAGCCT	45.0	50.0	58.3	58.1	829-845 bp	1.0	79.1
ycf3-trnS-GGA_p3	TTGGCCACGATTGAAAAGGT	ACGGAAAGAGAGGGATTCGA	45.0	50.0	58.3	58.2	850-866 bp	1.0	78.6
ycf3-trnS-GGA_p4	GGCCACGATTGAAAAGGTCT	TGCTACGCCTTTAACCACTC	50.0	50.0	58.5	57.9	792-808 bp	1.0	78.0
ycf3-trnS-GGA_p5	GGCCACGATTGAAAAGGTCT	CCCTCGGTAAACAAAAGCCT	50.0	50.0	58.5	58.1	827-843 bp	1.0	77.8