Hotspot

trnC-GCA-petN

LSC · 27979-28726 · 748 bp

Back to Myricaria

Pi 0.0115

MarkerSeek score 54.30

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 748
variable_sites: 23
pi: 0.0115
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	ATTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.9	58.2	796-808 bp	1.0	80.7
trnC-GCA-petN_p2	ATTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	57.9	57.6	857-869 bp	1.0	78.9
trnC-GCA-petN_p3	TAAGGCGGAGGATTGCAAAT	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.6	58.2	821-833 bp	1.0	78.3
trnC-GCA-petN_p4	GTAAGGCGGAGGATTGCAAA	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.5	58.2	822-834 bp	1.0	77.5
trnC-GCA-petN_p5	TAAGGCGGAGGATTGCAAAT	AGAGTCCACTTCTTCCCCAT	45.0	50.0	57.6	57.6	882-894 bp	1.0	76.5