Hotspot

trnS-GCU-trnG-UCC

LSC · 8172-9070 · 899 bp

Back to Myricaria

Pi 0.0178
MarkerSeek score 70.40
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
899
variable_sites
41
pi
0.0178
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CCCTCGGTACAAATGACTCG AATCAAACCGAAAGACCCCT 55.0 45.0 58.1 57.0 1016-1056 bp 1.0 78.8
trnS-GCU-trnG-UCC_p2 CCCTCGGTACAAATGACTCG ATCAAACCGAAAGACCCCTT 55.0 45.0 58.1 57.0 1015-1055 bp 1.0 78.8
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA AATCAAACCGAAAGACCCCT 50.0 45.0 58.2 57.0 1037-1077 bp 1.0 78.1
trnS-GCU-trnG-UCC_p4 ACGGAAAGAGAGGGATTCGA ATCAAACCGAAAGACCCCTT 50.0 45.0 58.2 57.0 1036-1076 bp 1.0 78.1
trnS-GCU-trnG-UCC_p5 CCCTCGGTACAAATGACTCG ACCGAAAGACCCCTTAACTC 55.0 50.0 58.1 56.9 1010-1050 bp 1.0 77.4