Hotspot

psbK-psbI

LSC · 7453-7861 · 409 bp

Back to Myricaria

Pi 0.0129

MarkerSeek score 61.20

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 409
variable_sites: 14
pi: 0.0129
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAAGCTGCGGTAAGTTTTCG	AGGATTACGCCCTGGATCAT	50.0	50.0	58.0	58.3	516-530 bp	1.0	81.9
psbK-psbI_p2	AGCTGCGGTAAGTTTTCGAT	AGGATTACGCCCTGGATCAT	45.0	50.0	57.9	58.3	514-528 bp	1.0	81.6
psbK-psbI_p3	AAGCTGCGGTAAGTTTTCGA	AGGATTACGCCCTGGATCAT	45.0	50.0	58.1	58.3	515-529 bp	1.0	81.3
psbK-psbI_p4	GCAAGCTGCGGTAAGTTTTC	AGGATTACGCCCTGGATCAT	50.0	50.0	58.3	58.3	517-531 bp	1.0	79.8
psbK-psbI_p5	CAAGCTGCGGTAAGTTTTCG	GGATTACGCCCTGGATCATT	50.0	50.0	58.0	57.4	515-529 bp	1.0	79.5