Hotspot

psbN

LSC · 74070-74201 · 132 bp

Back to Myosurus

Pi 0

MarkerSeek score 20.60

Primer available yes

Label psbN

Feature Metadata

label_name: psbN
feature_type: gene
region: LSC
length_bp: 132
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 20.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbN_p1	TGGTAGGCTCATTACTTTACTTCA	AGATCCATTGTGGTCTTGAACA	37.5	40.9	57.4	57.6	188 bp	1.0	40.0
psbN_p2	TGGTAGGCTCATTACTTTACTTCAA	AGATCCATTGTGGTCTTGAACA	36.0	40.9	58.0	57.6	188 bp	1.0	40.0
psbN_p3	TGGTAGGCTCATTACTTTACTTCA	GATCCATTGTGGTCTTGAACA	37.5	42.9	57.4	56.2	187 bp	1.0	40.0
psbN_p4	TGGTAGGCTCATTACTTTACTTCA	CCATTGTGGTCTTGAACATTGA	37.5	40.9	57.4	57.1	184 bp	1.0	40.0
psbN_p5	TGGTAGGCTCATTACTTTACTTCA	TCCATTGTGGTCTTGAACATTG	37.5	40.9	57.4	57.1	185 bp	1.0	40.0