Hotspot

ndhA

SSC · 118693-120844 · 2152 bp

Back to Myosurus

Pi 0.00467

MarkerSeek score 47.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2152
variable_sites: 10
pi: 0.00467
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACGCGAACACATACTTCACA	GCCGCTTTGATCGAACAAAA	45.0	45.0	57.9	58.0	2744-2754 bp	1.0	79.2
ndhA_p2	TTCAAAGTGGATTCGTCCCC	GCACTGTTGGTGGAGAAGAA	50.0	50.0	57.8	58.0	2964-2974 bp	1.0	78.8
ndhA_p3	GTCCCCGAAAACGTTCTGAT	GCACTGTTGGTGGAGAAGAA	50.0	50.0	58.2	58.0	2950-2960 bp	1.0	78.8
ndhA_p4	TCCCCGAAAACGTTCTGATG	GCACTGTTGGTGGAGAAGAA	50.0	50.0	58.2	58.0	2949-2959 bp	1.0	78.8
ndhA_p5	GACCGATATACCTTGCAGCC	GCACTGTTGGTGGAGAAGAA	55.0	50.0	58.2	58.0	2854-2864 bp	1.0	78.8