Hotspot

ndhE-ndhG

SSC · 116992-117237 · 246 bp

Back to Myosurus

Pi 0.0325

MarkerSeek score 71.20

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 246
variable_sites: 8
pi: 0.0325
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	AAGACACATAAGCGCTCGAA	TTAGTCGCCTTAATCGGTGC	45.0	50.0	57.6	58.1	393-395 bp	1.0	78.2
ndhE-ndhG_p2	AGACACATAAGCGCTCGAAC	TTAGTCGCCTTAATCGGTGC	50.0	50.0	58.4	58.1	392-394 bp	1.0	78.1
ndhE-ndhG_p3	CAAGACACATAAGCGCTCGA	TTAGTCGCCTTAATCGGTGC	50.0	50.0	58.4	58.1	394-396 bp	1.0	78.1
ndhE-ndhG_p4	TCAAGACACATAAGCGCTCG	TTAGTCGCCTTAATCGGTGC	50.0	50.0	58.4	58.1	395-397 bp	1.0	78.1
ndhE-ndhG_p5	AAGACACATAAGCGCTCGAA	TCGCCTTAATCGGTGCAATT	45.0	45.0	57.6	58.3	389-391 bp	1.0	76.7