Hotspot

ycf1-ndhF

SSC · 108290-109336 · 1047 bp

Back to Myosurus

Pi 0.0233

MarkerSeek score 73.50

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 1047
variable_sites: 24
pi: 0.0233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	GGGCGGATCTGGACAAAATA	TGGGGGCCGAATATCTTCTT	50.0	50.0	57.7	58.5	1163-1177 bp	1.0	76.1
ycf1-ndhF_p2	AGGAACAAGAGGGATCCACT	TGGGGGCCGAATATCTTCTT	50.0	50.0	57.6	58.5	1238-1252 bp	1.0	75.8
ycf1-ndhF_p3	CAGGAACAAGAGGGATCCAC	TGGGGGCCGAATATCTTCTT	55.0	50.0	57.6	58.5	1239-1253 bp	1.0	75.4
ycf1-ndhF_p4	GGGCGGATCTGGACAAAATA	ATATAGGTGGGGGCCGAATA	50.0	50.0	57.7	57.3	1170-1184 bp	1.0	74.7
ycf1-ndhF_p5	GGGCGGATCTGGACAAAATA	TATAGGTGGGGGCCGAATAT	50.0	50.0	57.7	57.3	1169-1183 bp	1.0	74.7