Hotspot

rps8-rpl14

LSC · 80239-80503 · 265 bp

Back to Myosurus

Pi 0.0151

MarkerSeek score 61.00

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 265
variable_sites: 4
pi: 0.0151
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	CGGCAATAGTATCCCTACCC	AAGGGAACTCGGGTTTTTGG	55.0	50.0	56.9	58.3	377 bp	1.0	70.0
rps8-rpl14_p2	CGGCAATAGTATCCCTACCC	CCAAAGGGAACTCGGGTTTT	55.0	50.0	56.9	58.3	380 bp	1.0	70.0
rps8-rpl14_p3	CGGCAATAGTATCCCTACCC	TCGCGAGTTGAGACAATTGA	55.0	45.0	56.9	57.6	348 bp	1.0	69.0
rps8-rpl14_p4	TCGGCAATAGTATCCCTACCC	AAGGGAACTCGGGTTTTTGG	52.4	50.0	58.5	58.3	378 bp	1.0	66.7
rps8-rpl14_p5	TCGGCAATAGTATCCCTACCC	CCAAAGGGAACTCGGGTTTT	52.4	50.0	58.5	58.3	381 bp	1.0	66.7