Hotspot

accD-rps12

LSC · 58319-59264 · 946 bp

Back to Myosurus

Pi 0.00425

MarkerSeek score 61.60

Primer available yes

Label accD-rps12

Feature Metadata

label_name: accD-rps12
feature_type: igs
region: LSC
length_bp: 946
variable_sites: 4
pi: 0.00425
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-rps12_p1	AGGCGGGATTATTCGACCTA	CTGAGGACATCCCCGAAGAT	50.0	55.0	58.0	58.6	1128-1138 bp	1.0	75.9
accD-rps12_p2	CAAGACGCTGAGCCCTTATT	CTGAGGACATCCCCGAAGAT	50.0	55.0	58.0	58.6	1150-1160 bp	1.0	75.7
accD-rps12_p3	GGCGGGATTATTCGACCTAC	CTGAGGACATCCCCGAAGAT	55.0	55.0	57.9	58.6	1127-1137 bp	1.0	75.3
accD-rps12_p4	ATTCGAGGCGGGATTATTCG	CTGAGGACATCCCCGAAGAT	50.0	55.0	57.9	58.6	1133-1143 bp	1.0	75.3
accD-rps12_p5	GTTTCCGAGGCCAACTGTAA	CTGAGGACATCCCCGAAGAT	50.0	55.0	58.1	58.6	1053-1063 bp	1.0	75.0