Hotspot

psbM-trnD-GUC

LSC · 27540-28131 · 592 bp

Back to Myosurus

Pi 0.0103

MarkerSeek score 66.50

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 592
variable_sites: 6
pi: 0.0103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GAACAGGGCAGTAGCAATGA	TCGCTGGGATTGTAGTTCAA	50.0	45.0	57.9	56.9	705-712 bp	1.0	72.0
psbM-trnD-GUC_p2	GAACAGGGCAGTAGCAATGA	TTCGCTGGGATTGTAGTTCA	50.0	45.0	57.9	56.9	706-713 bp	1.0	72.0
psbM-trnD-GUC_p3	GAATGAACAGGGCAGTAGCA	TCGCTGGGATTGTAGTTCAA	50.0	45.0	57.9	56.9	709-716 bp	1.0	72.0
psbM-trnD-GUC_p4	GAATGAACAGGGCAGTAGCA	TTCGCTGGGATTGTAGTTCA	50.0	45.0	57.9	56.9	710-717 bp	1.0	72.0
psbM-trnD-GUC_p5	TGAACAGGGCAGTAGCAATG	TCGCTGGGATTGTAGTTCAA	50.0	45.0	58.2	56.9	706-713 bp	1.0	71.5