Hotspot

trnS-GCU-trnG-UCC

LSC · 6978-7341 · 364 bp

Back to Myosurus

Pi 0.011
MarkerSeek score 58.80
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
364
variable_sites
4
pi
0.011
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA AACCGAAAGACCCCTTAACT 50.0 45.0 58.0 56.4 471 bp 1.0 67.6
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC AACCGAAAGACCCCTTAACT 50.0 45.0 58.0 56.4 470 bp 1.0 67.5
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA AAACCGAAAGACCCCTTAACT 50.0 42.9 58.0 57.1 472 bp 1.0 65.7
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC AAACCGAAAGACCCCTTAACT 50.0 42.9 58.0 57.1 471 bp 1.0 65.7
trnS-GCU-trnG-UCC_p5 GTCCACTCAGCCATCTTTCC AACCGAAAGACCCCTTAACT 55.0 45.0 58.3 56.4 456 bp 1.0 65.6