Hotspot

rpl33

LSC · 65733-65939 · 207 bp

Back to Myosotis

Pi 0.00246

MarkerSeek score 26.20

Primer available yes

Label rpl33

Feature Metadata

label_name: rpl33
feature_type: gene
region: LSC
length_bp: 207
variable_sites: 2
pi: 0.00246
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 26.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33_p1	ATGCGTTGACATTCCCCTTT	TGAAGAAAGAGTAAGGCGGC	45.0	50.0	58.1	57.9	1083-1094 bp	1.0	80.8
rpl33_p2	ATGCGTTGACATTCCCCTTT	CAATTCGATCCCCCGATTCA	45.0	50.0	58.1	57.7	843-854 bp	1.0	80.1
rpl33_p3	AGGAGGGTTTTGAATGCGAG	TGAAGAAAGAGTAAGGCGGC	50.0	50.0	58.2	57.9	1199-1210 bp	1.0	79.8
rpl33_p4	GGAGGGTTTTGAATGCGAGA	TGAAGAAAGAGTAAGGCGGC	50.0	50.0	58.2	57.9	1198-1209 bp	1.0	79.7
rpl33_p5	AAGGAGGGTTTTGAATGCGA	TGAAGAAAGAGTAAGGCGGC	45.0	50.0	57.7	57.9	1200-1211 bp	1.0	78.9