Hotspot

atpA

LSC · 9915-11438 · 1524 bp

Back to Myosotis

Pi 0.000561

MarkerSeek score 34.50

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 3
pi: 0.000561
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	AGCCTTCCAAGCTAACGATG	GTAATTGAGCGTGAGAGCCA	50.0	50.0	58.0	58.0	2453-2477 bp	1.0	80.7
atpA_p2	AGCCTTCCAAGCTAACGATG	GTGGAAATAGAAGCCGAGCA	50.0	50.0	58.0	58.0	2230-2254 bp	1.0	80.7
atpA_p3	CCTAGCCTTCCAAGCTAACG	GTAATTGAGCGTGAGAGCCA	55.0	50.0	58.1	58.0	2456-2480 bp	1.0	80.5
atpA_p4	AGCCTTCCAAGCTAACGATG	TTGGGGGCGCTAAAAGAAAT	50.0	45.0	58.0	58.1	1966-1990 bp	1.0	80.5
atpA_p5	CCTAGCCTTCCAAGCTAACG	GTGGAAATAGAAGCCGAGCA	55.0	50.0	58.1	58.0	2233-2257 bp	1.0	80.4