Hotspot

rps18-rpl20

LSC · 66395-66659 · 265 bp

Back to Myosotis

Pi 0.01

MarkerSeek score 60.90

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 265
variable_sites: 10
pi: 0.01
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	ACTGCCATAAAACAAGCTCG	AACGAAGTAGAATCACCGGA	45.0	45.0	56.7	56.3	388-418 bp	0.909091	58.4
rps18-rpl20_p2	ACTGCCATAAAACAAGCTCG	ACGAAGTAGAATCACCGGAA	45.0	45.0	56.7	56.3	387-417 bp	0.909091	58.4
rps18-rpl20_p3	CTGCCATAAAACAAGCTCGT	AACGAAGTAGAATCACCGGA	45.0	45.0	56.7	56.3	387-417 bp	0.909091	58.4
rps18-rpl20_p4	CTGCCATAAAACAAGCTCGT	ACGAAGTAGAATCACCGGAA	45.0	45.0	56.7	56.3	386-416 bp	0.909091	58.4
rps18-rpl20_p5	ACTGCCATAAAACAAGCTCGT	ACGAAGTAGAATCACCGGAA	42.9	45.0	58.5	56.3	387-417 bp	0.909091	56.7