Hotspot

accD-psaI

LSC · 57127-57683 · 557 bp

Back to Myosotis

Pi 0.00317

MarkerSeek score 56.80

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 557
variable_sites: 5
pi: 0.00317
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	ATAGTATGCGCTTTTGGGGG	GCCATTGAAATTGCCGGAAA	50.0	45.0	58.0	57.9	678-715 bp	1.0	83.7
accD-psaI_p2	ATAGTATGCGCTTTTGGGGG	GAAGCCATTGAAATTGCCGG	50.0	50.0	58.0	58.4	681-718 bp	1.0	81.6
accD-psaI_p3	ATAGTATGCGCTTTTGGGGG	GCCGGAAATACTAGGCCTAC	50.0	55.0	58.0	57.5	666-703 bp	1.0	80.8
accD-psaI_p4	ATAGTATGCGCTTTTGGGGG	AGAAGCCATTGAAATTGCCG	50.0	45.0	58.0	57.3	682-719 bp	1.0	79.1
accD-psaI_p5	ATAGTATGCGCTTTTGGGGG	AGCCATTGAAATTGCCGGAA	50.0	45.0	58.0	58.7	679-716 bp	1.0	78.5