Hotspot

tRNA-Thr-tRNA-Leu

LSC · 45877-46583 · 707 bp

Back to Myosotis

Pi 0.00332
MarkerSeek score 60.00
Primer available yes
Label tRNA-Thr-tRNA-Leu

Feature Metadata

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
tRNA-Thr-tRNA-Leu_p1 AAAATGGGAAAAGCCGGCTA ACCAATTTCGCCATATCCCC 45.0 50.0 58.1 58.0 806-841 bp 1.0 81.2
tRNA-Thr-tRNA-Leu_p2 GCGATGCTCTAACCTCTGAG ACCAATTTCGCCATATCCCC 55.0 50.0 57.9 58.0 752-787 bp 1.0 80.8
tRNA-Thr-tRNA-Leu_p3 AAAATGGGAAAAGCCGGCTA TAGCGTCTACCAATTTCGCC 45.0 50.0 58.1 58.1 814-849 bp 1.0 80.6
tRNA-Thr-tRNA-Leu_p4 AAATGGGAAAAGCCGGCTAT ACCAATTTCGCCATATCCCC 45.0 50.0 57.8 58.0 805-840 bp 1.0 80.5
tRNA-Thr-tRNA-Leu_p5 GCGATGCTCTAACCTCTGAG TAGCGTCTACCAATTTCGCC 55.0 50.0 57.9 58.1 760-795 bp 1.0 80.3