Hotspot

pafI-tRNA-Ser

LSC · 43572-44463 · 892 bp

Back to Myosotis

Pi 0.00433

MarkerSeek score 62.50

Primer available yes

Label pafI-tRNA-Ser

Feature Metadata

label_name: pafI-tRNA-Ser
feature_type: igs
region: LSC
length_bp: 892
variable_sites: 16
pi: 0.00433
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafI-tRNA-Ser_p1	CATTTATCCGCGATCTAGGCA	AAGGTTCGGAAAGAGAGGGA	47.6	50.0	58.0	58.0	991-1036 bp	1.0	74.5
pafI-tRNA-Ser_p2	CATTTATCCGCGATCTAGGCA	CCCTCGGTAAACAAAAGCCT	47.6	50.0	58.0	58.1	965-1010 bp	1.0	74.0
pafI-tRNA-Ser_p3	CATTTATCCGCGATCTAGGCA	TCGGAAAGAGAGGGATTCGA	47.6	50.0	58.0	57.9	986-1031 bp	1.0	73.6
pafI-tRNA-Ser_p4	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	47.6	45.0	58.0	57.8	936-981 bp	1.0	73.3
pafI-tRNA-Ser_p5	CATTTATCCGCGATCTAGGCA	AGGTTCGGAAAGAGAGGGAT	47.6	50.0	58.0	57.7	990-1035 bp	1.0	72.6