Hotspot

psaA-pafI

LSC · 40782-41596 · 815 bp

Back to Myosotis

Pi 0.0017

MarkerSeek score 59.80

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 815
variable_sites: 6
pi: 0.0017
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	GTTCTTGAAAAATGGCCCGG	ATTGCAGAGGCTTGGTTTGA	50.0	45.0	57.9	58.0	1034-1058 bp	1.0	81.5
psaA-pafI_p2	GTTCTTGAAAAATGGCCCGG	TTGCAGAGGCTTGGTTTGAT	50.0	45.0	57.9	58.0	1033-1057 bp	1.0	81.5
psaA-pafI_p3	GCCCATTCCTCGAAAGAAGT	ATTGCAGAGGCTTGGTTTGA	50.0	45.0	57.9	58.0	1010-1034 bp	1.0	81.3
psaA-pafI_p4	GCCCATTCCTCGAAAGAAGT	TTGCAGAGGCTTGGTTTGAT	50.0	45.0	57.9	58.0	1009-1033 bp	1.0	81.3
psaA-pafI_p5	GTTCTTGAAAAATGGCCCGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	57.9	58.0	1015-1039 bp	1.0	81.2