Hotspot

tRNA-Ser-tRNA-Ser

LSC · 8076-8860 · 785 bp

Back to Myosotis

Pi 0.00617

MarkerSeek score 69.80

Primer available yes

Label tRNA-Ser-tRNA-Ser

Feature Metadata

label_name: tRNA-Ser-tRNA-Ser
feature_type: igs
region: LSC
length_bp: 785
variable_sites: 19
pi: 0.00617
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ser-tRNA-Ser_p1	ATTAGCAATCCGCCGCTTTA	TGAATCAAACCGAAAGACCCT	45.0	42.9	58.0	57.5	899-921 bp	1.0	72.3
tRNA-Ser-tRNA-Ser_p2	TAGCAATCCGCCGCTTTAAT	TGAATCAAACCGAAAGACCCT	45.0	42.9	58.0	57.5	897-919 bp	1.0	72.3
tRNA-Ser-tRNA-Ser_p3	ACGGAAAGAGAGGGATTCGA	TGAATCAAACCGAAAGACCCT	50.0	42.9	58.2	57.5	948-970 bp	1.0	71.2
tRNA-Ser-tRNA-Ser_p4	TTAGCAATCCGCCGCTTTAA	TGAATCAAACCGAAAGACCCT	45.0	42.9	58.3	57.5	898-920 bp	1.0	70.6
tRNA-Ser-tRNA-Ser_p5	ACAACAACGGAAAGAGAGGG	TGAATCAAACCGAAAGACCCT	50.0	42.9	57.7	57.5	954-976 bp	1.0	70.3