Hotspot

psbA

LSC · 411-1510 · 1100 bp

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Pi 0.00188

MarkerSeek score 63.20

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1100
variable_sites: 2
pi: 0.00188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	AAGGAGCAATAACGCCTTCT	ATTTGTGTGCTTGGGAGTCC	45.0	50.0	57.2	58.4	1212-1250 bp	1.0	73.3
psbA_p2	AGGAGCAATAACGCCTTCTT	ATTTGTGTGCTTGGGAGTCC	45.0	50.0	57.2	58.4	1211-1249 bp	1.0	73.3
psbA_p3	AAGGAGCAATAACGCCTTCTT	ATTTGTGTGCTTGGGAGTCC	42.9	50.0	57.9	58.4	1212-1250 bp	1.0	71.0
psbA_p4	AAGGAGCAATAACGCCTTCT	TTTGTGTGCTTGGGAGTCC	45.0	52.6	57.2	57.9	1211-1249 bp	1.0	67.4
psbA_p5	AGGAGCAATAACGCCTTCTT	TTTGTGTGCTTGGGAGTCC	45.0	52.6	57.2	57.9	1210-1248 bp	1.0	67.4