Hotspot

trnI-GAU-rrn16

IRa · 137478-137779 · 302 bp

Back to Mussaenda

Pi 0.0373

MarkerSeek score 76.10

Primer available yes

Label trnI-GAU-rrn16

Feature Metadata

label_name: trnI-GAU-rrn16
feature_type: igs
region: IRa
length_bp: 302
variable_sites: 11
pi: 0.0373
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-GAU-rrn16_p1	GGCACAACGACGCAATTATC	CAAGGTAGCCGTACTGGAAG	50.0	55.0	58.2	57.7	389-396 bp	1.0	79.8
trnI-GAU-rrn16_p2	CTATCCATGTGGCTGAGAGC	CAAGGTAGCCGTACTGGAAG	55.0	55.0	57.8	57.7	421-428 bp	1.0	79.7
trnI-GAU-rrn16_p3	GGCACAACGACGCAATTATC	AGTGACTGGAGTGAAGTCGT	50.0	50.0	58.2	58.3	411-418 bp	1.0	79.5
trnI-GAU-rrn16_p4	CTATCCATGTGGCTGAGAGC	AGTGACTGGAGTGAAGTCGT	55.0	50.0	57.8	58.3	443-450 bp	1.0	79.4
trnI-GAU-rrn16_p5	CTATCCATGTGGCTGAGAGC	AAGTCGTAACAAGGTAGCCG	55.0	50.0	57.8	57.6	430-437 bp	1.0	79.1