Hotspot

rps15-ycf1

SSC · 124656-125041 · 386 bp

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Pi 0.00272

MarkerSeek score 60.60

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 386
variable_sites: 1
pi: 0.00272
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCCAAATGTGAAGTAAGCCTCT	CGGCAGTCGTTTCAGTATGT	40.9	50.0	57.4	58.3	540-557 bp	1.0	59.5
rps15-ycf1_p2	CCAAATGTGAAGTAAGCCTCTC	CGGCAGTCGTTTCAGTATGT	45.5	50.0	57.0	58.3	539-556 bp	1.0	57.0
rps15-ycf1_p3	TTCCAAATGTGAAGTAAGCCTCT	CGGCAGTCGTTTCAGTATGT	39.1	50.0	58.0	58.3	541-558 bp	1.0	56.5
rps15-ycf1_p4	TCCAAATGTGAAGTAAGCCTC	CGGCAGTCGTTTCAGTATGT	42.9	50.0	55.9	58.3	540-557 bp	1.0	55.6
rps15-ycf1_p5	CCAAATGTGAAGTAAGCCTCT	CGGCAGTCGTTTCAGTATGT	42.9	50.0	55.9	58.3	539-556 bp	1.0	55.5