Hotspot

rpl32-trnL-UAG

SSC · 114684-115389 · 706 bp

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Pi 0.179

MarkerSeek score 96.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 706
variable_sites: 110
pi: 0.179
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 96.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGGGGTATTGGACAGCCTTA	TATCATGCCCTTTTCGGACG	50.0	50.0	57.7	58.1	1110-1242 bp	1.0	88.8
rpl32-trnL-UAG_p2	AGGGGTATTGGACAGCCTTA	AGCCTTGGGTAAAAACAGCA	50.0	45.0	57.7	57.9	1306-1438 bp	1.0	87.5
rpl32-trnL-UAG_p3	AGGGGTATTGGACAGCCTTA	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	57.7	58.1	1316-1448 bp	1.0	87.4
rpl32-trnL-UAG_p4	AGGGGTATTGGACAGCCTTA	TGAAGTAGCAAAGCCTTGGG	50.0	50.0	57.7	58.1	1317-1449 bp	1.0	87.4
rpl32-trnL-UAG_p5	AGGGGTATTGGACAGCCTTA	GCCTTGGGTAAAAACAGCAC	50.0	50.0	57.7	57.9	1305-1437 bp	1.0	87.0