Hotspot

ndhF-rpl32

SSC · 113645-114521 · 877 bp

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Pi 0.0107

MarkerSeek score 69.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 877
variable_sites: 9
pi: 0.0107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAAAGAAGAAGTCCCAGCCC	TAAGGCTGTCCAATACCCCT	50.0	50.0	57.7	57.7	1011-1050 bp	1.0	79.1
ndhF-rpl32_p2	AAGAAGAAGTCCCAGCCCTA	TAAGGCTGTCCAATACCCCT	50.0	50.0	57.7	57.7	1009-1048 bp	1.0	78.7
ndhF-rpl32_p3	AAAGAAGAAGTCCCAGCCCT	TAAGGCTGTCCAATACCCCT	50.0	50.0	58.5	57.7	1010-1049 bp	1.0	77.1
ndhF-rpl32_p4	AGAAGAAGTCCCAGCCCTAT	TAAGGCTGTCCAATACCCCT	50.0	50.0	57.4	57.7	1008-1047 bp	1.0	76.9
ndhF-rpl32_p5	AAAAGAAGAAGTCCCAGCCC	AAGGCTGTCCAATACCCCTT	50.0	50.0	57.7	58.6	1010-1049 bp	1.0	76.4