Hotspot

accD-psaI

LSC · 59936-60698 · 763 bp

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Pi 0.0697

MarkerSeek score 92.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 763
variable_sites: 48
pi: 0.0697
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 92.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GACCTAATCGTACCGCGTAA	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.2	58.0	875-932 bp	1.0	82.0
accD-psaI_p2	GACCTAATCGTACCGCGTAA	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.2	57.9	881-938 bp	1.0	81.3
accD-psaI_p3	ATTTCAGTTCCACGCTTTCG	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.0	58.0	828-885 bp	1.0	80.5
accD-psaI_p4	ATTTCAGTTCCACGCTTTCG	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.0	57.9	834-891 bp	1.0	79.9
accD-psaI_p5	GACCTAATCGTACCGCGTAA	GCCGGAAATACTAGGCCTAC	50.0	55.0	57.2	57.5	864-921 bp	1.0	78.6