Hotspot

atpB-rbcL

LSC · 55494-56282 · 789 bp

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Pi 0.00392

MarkerSeek score 63.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 789
variable_sites: 3
pi: 0.00392
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CCCCGGAACCAGAAGTAGTA	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	848-872 bp	1.0	73.2
atpB-rbcL_p2	GCCGATGATTTGGACAATGC	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.2	58.0	898-922 bp	1.0	73.0
atpB-rbcL_p3	CCCCGGAACCAGAAGTAGTA	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.1	58.1	847-871 bp	1.0	73.0
atpB-rbcL_p4	GCCGATGATTTGGACAATGC	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.2	58.1	897-921 bp	1.0	72.7
atpB-rbcL_p5	AGGCTACATCTAGTACCGGG	CACCAGCTTTGAATCCAACAC	55.0	47.6	57.7	58.0	917-941 bp	1.0	71.9