Hotspot

petN-psbM

LSC · 28819-30011 · 1193 bp

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Pi 0.00345

MarkerSeek score 63.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1193
variable_sites: 4
pi: 0.00345
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	TCATTCTAGTTCCTACGGCCT	50.0	47.6	58.0	58.0	1257-1289 bp	1.0	74.0
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	TCATTCTAGTTCCTACGGCCT	50.0	47.6	58.0	58.0	1255-1287 bp	1.0	74.0
petN-psbM_p3	TCGTAGTGTGGGGAAGAAGT	TCTAGTTCCTACGGCCTTTCT	50.0	47.6	58.0	57.9	1251-1283 bp	1.0	73.5
petN-psbM_p4	ACTCGTAGTGTGGGGAAGAA	TCTAGTTCCTACGGCCTTTCT	50.0	47.6	58.0	57.9	1253-1285 bp	1.0	73.4
petN-psbM_p5	CGTAGTGTGGGGAAGAAGTG	TCATTCTAGTTCCTACGGCCT	55.0	47.6	57.9	58.0	1254-1286 bp	1.0	73.4