Hotspot

rps15-ycf1

SSC · 122780-123224 · 445 bp

Back to Muscari

Pi 0.019

MarkerSeek score 63.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 445
variable_sites: 53
pi: 0.019
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CGAATACCCAACCGACCAAT	ACGCAGCAATACACTTGTGA	50.0	45.0	58.0	58.1	902-1084 bp	1.0	90.4
rps15-ycf1_p2	TTTCCAATCGTTCTGGTGCA	ACGCAGCAATACACTTGTGA	45.0	45.0	58.0	58.1	1297-1479 bp	1.0	89.6
rps15-ycf1_p3	CGAATACCCAACCGACCAAT	ATTCCCGGAATTGGCATGTT	50.0	45.0	58.0	58.1	792-974 bp	0.974359	88.6
rps15-ycf1_p4	TTTCCAATCGTTCTGGTGCA	AATTCCCGGAATTGGCATGT	45.0	45.0	58.0	58.1	1188-1370 bp	0.974359	87.9
rps15-ycf1_p5	TTTCCAATCGTTCTGGTGCA	ATTCCCGGAATTGGCATGTT	45.0	45.0	58.0	58.1	1187-1369 bp	0.974359	87.9