Hotspot

ccsA-ndhD

SSC · 114522-114752 · 231 bp

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Pi 0.0255

MarkerSeek score 69.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 231
variable_sites: 30
pi: 0.0255
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ACGAATTCGGCATTTGTAGC	CCGATTTCGTTCTCTCGCTA	45.0	50.0	57.2	57.5	401-433 bp	0.923077	77.3
ccsA-ndhD_p2	ACGAATTCGGCATTTGTAGC	ATCCCGATTTCGTTCTCTCG	45.0	50.0	57.2	57.5	404-436 bp	0.923077	77.0
ccsA-ndhD_p3	CGAATTCGGCATTTGTAGCT	CCGATTTCGTTCTCTCGCTA	45.0	50.0	56.9	57.5	400-432 bp	0.923077	75.0
ccsA-ndhD_p4	CGAATTCGGCATTTGTAGCT	ATCCCGATTTCGTTCTCTCG	45.0	50.0	56.9	57.5	403-435 bp	0.923077	74.7
ccsA-ndhD_p5	ACGAATTCGGCATTTGTAGC	CCGATTTCGTTCTCTCGCTAT	45.0	47.6	57.2	58.0	401-433 bp	0.923077	73.0