Hotspot

rpl32-trnL-UAG

SSC · 112589-113397 · 809 bp

Back to Muscari

Pi 0.018
MarkerSeek score 74.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
809
variable_sites
100
pi
0.018
unique_haplotype_count
35
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCAAAGTCAATTTCCACCGG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.9 57.9 920-1007 bp 1.0 90.5
rpl32-trnL-UAG_p2 GCAAAGTCAATTTCCACCGG AGCAGCGTGTCTACCAATTT 50.0 45.0 57.9 57.8 913-1000 bp 1.0 90.0
rpl32-trnL-UAG_p3 GCAAAGTCAATTTCCACCGG CCTAAGAGCAGCGTGTCTAC 50.0 55.0 57.9 57.8 919-1006 bp 1.0 89.9
rpl32-trnL-UAG_p4 GCAAAGTCAATTTCCACCGG CTAAGAGCAGCGTGTCTACC 50.0 55.0 57.9 57.8 918-1005 bp 1.0 89.9
rpl32-trnL-UAG_p5 GCAAAGTCAATTTCCACCGG GAGCAGCGTGTCTACCAATT 50.0 50.0 57.9 58.3 914-1001 bp 1.0 89.3