Hotspot

ndhF-rpl32

SSC · 111503-112414 · 912 bp

Back to Muscari

Pi 0.0144

MarkerSeek score 67.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 912
variable_sites: 101
pi: 0.0144
unique_haplotype_count: 37
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CACTCGGAAAAGCCCACATA	CCGGTGGAAATTGACTTTGC	50.0	50.0	57.9	57.9	913-1259 bp	1.0	90.5
ndhF-rpl32_p2	ACTCGGAAAAGCCCACATAC	CCGGTGGAAATTGACTTTGC	50.0	50.0	57.9	57.9	912-1258 bp	1.0	90.5
ndhF-rpl32_p3	CAAAACACTCGGAAAAGCCC	CCGGTGGAAATTGACTTTGC	50.0	50.0	57.9	57.9	918-1264 bp	1.0	90.2
ndhF-rpl32_p4	TTTTTGACAAGCACTTGCCG	CCGGTGGAAATTGACTTTGC	45.0	50.0	58.1	57.9	1311-1657 bp	1.0	89.4
ndhF-rpl32_p5	GGGAATTGTGCAGATTTCGC	CCGGTGGAAATTGACTTTGC	50.0	50.0	58.1	57.9	1768-1773 bp	0.076923	41.9