Hotspot

psbE-petL

LSC · 63992-65010 · 1019 bp

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Pi 0.0112

MarkerSeek score 67.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1019
variable_sites: 89
pi: 0.0112
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCAGCCAATAGAAAACCGA	50.0	45.0	57.9	57.5	1061-1445 bp	1.0	85.9
psbE-petL_p2	ACCAACCCGCAATGAATAGG	AGCAGCCAATAGAAAACCGA	50.0	45.0	58.2	57.5	1063-1447 bp	1.0	84.4
psbE-petL_p3	ACCCGCAATGAATAGGGAAG	AGCAGCCAATAGAAAACCGA	50.0	45.0	57.7	57.5	1059-1443 bp	1.0	83.5
psbE-petL_p4	CCGCAATGAATAGGGAAGGT	AGCAGCCAATAGAAAACCGA	50.0	45.0	57.7	57.5	1057-1441 bp	1.0	83.5
psbE-petL_p5	CCCGCAATGAATAGGGAAGG	AGCAGCCAATAGAAAACCGA	55.0	45.0	58.4	57.5	1058-1442 bp	1.0	83.2